![]() The annual global production of citrus EO is approximately 16,000 tons, and the cost is about $14,000/ton on the international market. Citrus Eos are the main aromatic by-products of the juice extraction industry and are widely used in food, cosmetics and pharmaceutical industry. In the past few decades, plant essential oils (EOs) have attracted a lot of interest due to their safety and pharmacological properties including bacteriostatic, free radical scavenging, anti-inflammatory, and inhibitory effects on malignant tumor cell proliferation. Plant extracts have been used since ancient times, and now the focus is on their role in health promotion and their treatment and prevention properties for various diseases. With the frequent occurrence of food safety issues and the toxicity of synthetic chemicals, the demand for safe and natural alternatives is growing. The CCK-8 assay of cell proliferation showed that the light phase oil had a good inhibitory effect on the proliferation of HepG2 liver cancer cells and HCT116 colon cancer cells. DPPH and ABTS assays demonstrated that the light phase oil had good antioxidant activity. The light phase oil had the best antimicrobial effect on Bacillus subtilis, followed by Escherichia coli, Staphylococcus aureus and Salmonellaty phimurium. Twenty-four components were detected with a total relative content of 99.74%, including 97.48% of terpenes and 1.66% of oxygenated terpenes. The antimicrobial activity of the light phase oil was tested by filter paper diffusion method, and the anticancer activity was determined by the Cell Counting Kit-8 (CCK-8) assay. In this study, a light phase oil was obtained by molecular distillation from cold-pressed grapefruit essential oil and GC-MS was used to identify its chemical composition. ![]() However, bioactivity of its molecular distillate has not been well studied. Grapefruit essential oil has been proven to have wide range of bioactivities.
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